专利名称：DETECTION OF NUCLEIC ACIDS BY TARGET-CATALYZED PRODUCT FORMATION
发明人：ULLMAN, Edwin, F.,WESTERN, Linda, M.,ROSE,
Samuel, J.
申请号：EP95944648.0申请日：19951222公开号：EP0842294A2公开日：19980520
摘要： DESCRIBED a method for modifying an oligonucleotide, said PROCEDURE Anapplication in detecting an analyte polynucleotide. An oligonucleotide reversiblyhybridizes with a polynucleotide, FOR EXAMPLE, an analyte polynucleotide, in thepresence of 5 isothermal conditions. Analyte polynucleotide acts as a RECOGNITION TOALLOW A 5 cleaves Oligonucleotide TO SUPPLY (I) a first fragment that is substantiallyhybridizable with the analyte polynucleotide, and (ii) a second fragment ARE 3 withrespect to the first FRAGMENTO (oligonucleotide INTACT IN) and which is substantiallyhybridizable with the analyte polynucleotide. YOU GET AT LEAST 100 TIMES IN EXCESSOF MOLAR first fragment and / or second piece in relation to the amount of analytepolynucleotide MOLAR. It DETECTED THE PRESENCE OF THE FIRST PIECE AND / OR THESECOND EXTRACT, INDICATING PRESENCE the presence of analyte polynucleotide.PROCEDURE finds particular application in the detection of an analyte polynucleotide,such as DNA. ALSO DESCRIBED test kits TO IMPLEMENT PROCEDURES the presentinvention.
申请人：Behring Diagnostics GmbH
地址：Postfach 11 49 35001 Marburg DE
国籍：DE
代理机构：Zounek, Nikolai, Dipl.-Ing.
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