Method of isolating restriction fragment deletions

专利名称：Method of isolating restriction fragment

deletions in vibrio cholerae and productsthereof

发明人：James B. Kaper,Myron M. Levine申请号：US07/363383申请日：190605公开号：US049353A公开日：19900619

摘要：This invention relates to a method of isolating deletion mutants of Vibriocholerae, wherein the deletion is predetermined by digestion with restriction

endonucleases of known specificity. The deletions are inserted into the Vibrio choleraechromosome by in vivo recombination betweem a plasmid carrying the desired deletion,with adjacent flanking sequences, and the Vibrio cholerae chromosome. The inventionincludes the isolation and characterization of a new Vibrio cholerae strain having adeletion in the tox gene, as defined by Acc I restriction endonuclease sites.

申请人：SWISS SERUM AND VACCINE INSTITUTE BERNE

代理人：Bradford E. Kile,Kevin M. O'Brien,Ruffin B. Cordell

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